This protocol describes gating procedures to check T cells in mouse thymus, spleen, and blood. We described different T cell populations dependant on extracellular and intracellular markers. Discard the supernatant, resuspend cell pellets with 200 μL 1× D-PBS and Keep to the same process of extracellular staining with antibodies as https://kaiserlichesinterieur.de/2024/04/29/entdecken-sie-die-kraft-der-liebe-6-moglichkeiten-mehr-liebe-in-ihrem-leben-zu-kultivieren/